The novel follows Mma Precious Ramotswe, owner of the No. 1 Ladies' Detective Agency in Gaborone, Botswana, as she juggles two investigations while contending with the temporary absence of her indispensable assistant, Mma Makutsi. In Setswana, Botswana's national language, "Mma" and "Rra" are respectful titles for women and men.
The story opens with Mma Ramotswe reflecting on the unchanging nature of her small business and the people around her. Her husband, Mr. J.L.B. Matekoni, widely regarded as Botswana's finest mechanic, still runs his garage from the same premises the agency shares. Charlie, a perpetual apprentice at the garage, still spends his lunch hours watching girls. One thing, however, is changing: Mma Makutsi is visibly pregnant but refuses to discuss maternity leave, fearing that talking about the pregnancy prematurely could jinx the outcome. Months pass with the issue unresolved.
A new case arrives when Mma Sheba Kutso, an attorney specializing in wills and estates, visits the agency. Her late client, Rra Edgar Molapo, a respected cattle farmer whose father was a friend of Seretse Khama, Botswana's founding president, left his farm to his nephew Liso Molapo, the son of his deceased brother. A young man has come forward claiming to be Liso, presenting a passport and birth certificate, and Rra Edgar's sister, who lives on the farm, confirms his identity. Despite this documentation, Mma Sheba insists she feels "in her bones" that the young man is an imposter and asks Mma Ramotswe to prove it. Mma Ramotswe finds it odd that a lawyer would rely on intuition but agrees to investigate.
Meanwhile, Mma Makutsi and her husband, Phuti Radiphuti, face a cobra in their new house on the edge of town. Phuti borrows a snake-catching dog, which kills a snake in the bedroom. That evening, Mma Makutsi realizes the dead snake is much smaller than the cobra she saw under the bed, meaning the larger snake is still inside. As she turns to warn Phuti, sudden pain doubles her over: She has gone into premature labor, three weeks early.
Phuti arrives at the office the next morning to announce the birth of a boy. Mma Ramotswe is privately moved, remembering her own baby by her former husband, Note Mokoti, a child who lived only a short time before dying. Phuti shares that the baby's second name will be Clovis, after Clovis Andersen, author of
The Principles of Private Detection, the book that has guided Mma Ramotswe's career. Mma Makutsi wants only a few days of maternity leave, planning to return with a household helper to care for the baby.
A second case emerges when Mma Ramotswe encounters Mma Soleti, owner of the newly opened Minor Adjustment Beauty Salon. Mma Soleti confides that she received a parcel containing a feather from a ground hornbill, a bird widely believed to be an omen of death. The situation worsens when a whispering campaign spreads a false rumor that a customer's face "peeled off" during treatment, and anonymous leaflets devastate the salon's business. Mma Soleti identifies Daisy Manchwe, whose husband Mma Soleti had taken, as a potential enemy.
When Mma Ramotswe visits the new baby at the Radiphuti house, she must push past Phuti's obstructive aunt, who has moved in to enforce traditional seclusion customs. Mma Makutsi secretly takes Mma Ramotswe to the nursery, where she is deeply moved, reflecting on her assistant's long journey from poverty in Bobonong to this moment of motherhood.
Without Mma Makutsi, Mma Ramotswe investigates the Molapo case alone. At the farm, she meets Mma Molapo, Rra Edgar's sister, and Liso, a polite young man who speaks fluent Setswana and shares convincing memories of farm life and schooling in Swaziland. However, Mma Molapo repeatedly answers questions on Liso's behalf, steering the conversation. Mma Ramotswe leaves uncertain. A crucial clue surfaces when her friend Gwithie, who lives nearby at Mokolodi, mentions that Liso recently referred to his aunt as "my mother" before correcting himself. This suggests Mma Molapo may actually be Liso's mother, with a motive to substitute her own son for the real nephew, though Mma Ramotswe acknowledges the slip could be innocent.
Feeling the weight of working alone, Mma Ramotswe experiences a rare emotional low and is found crying at her desk by Mr. J.L.B. Matekoni. Concerned, he visits Mma Potokwane, the formidable matron of the local orphan farm, who diagnoses exhaustion and recommends he attend a "How to Be a Modern Husband" course. He reluctantly enrolls but endures only one uncomfortable session before slipping away, resolving to be more helpful in his own way. Later, Mma Ramotswe assures him he does not need to return, telling him that being a kind husband matters more than being a modern one.
Mma Ramotswe consults Mma Makutsi at home, and her assistant suggests examining the will itself. At the office of the Master of the High Court, Mma Ramotswe discovers a critical provision: The residue of the estate goes to "my good friend, Mma Sheba Kutso." If the named beneficiary cannot inherit because he is deceased, the legacy falls into the residue. Mma Sheba would get the farm if Liso is disqualified, confirming that the lawyer has a personal financial motive for hiring the agency.
The full truth emerges at Mma Potokwane's orphan farm, where a housemother named Mmamodise, who formerly cooked on the Molapo farm, inadvertently reveals the family secret. The boy on the farm is not Rra Edgar's nephew but his own son, born of an encounter between Rra Edgar and his teenage sister. Their father sent the sister away to give birth, and the baby was raised by strangers. The real nephew died in the same car accident that killed Rra Edgar's brother, but the sister kept this from Rra Edgar. She later brought her own son to the farm, passing him off as the Swazi nephew. Rra Edgar accepted and loved the boy without ever knowing the truth. The boy himself does not know his parentage. Mma Ramotswe promises Mmamodise the secret will remain safe.
On the Soleti case, Mma Makutsi returns to work with baby Itumelang in a portable crib and makes a breakthrough. Using a magnifying glass, she discovers that the threatening leaflet and a price list from Manchwe's photocopy shop, Clear Image Copies, bear the same distinctive mark from the same copier. They initially suspect Violet Sephotho, Mma Makutsi's longtime nemesis from the Botswana Secretarial College, since Manchwe's part-time assistant also works at Violet's shop. However, a salon trainee named Angela reveals that Manchwe had tried to bribe a property agent to take over Mma Soleti's lease for her own business. During an earlier visit, Mma Ramotswe and Mma Makutsi had interviewed Manchwe, who convincingly denied any hostility and claimed to be grateful that Mma Soleti took her unfaithful husband. In truth, Manchwe was behind the campaign all along. Mma Ramotswe devises a fitting consequence: Manchwe must produce free advertising leaflets for the salon. She also privately advises Mma Soleti to reflect on her own role in taking Manchwe's husband.
With both cases resolved, Mma Ramotswe and Mma Makutsi agree that the boy on the farm is the person Rra Edgar intended to inherit when he wrote "my nephew." They will tell Mma Sheba that Liso is the young man Rra Edgar had in mind, which is entirely true, without disclosing the family secret. On the Radiphuti verandah at sunset, Mma Ramotswe tells Mma Makutsi she has decided to make her a full partner in the No. 1 Ladies' Detective Agency, sharing in whatever profit or loss may come. Mma Makutsi replies that half of nothing is better than nothing of nothing and tells Mma Ramotswe she is the best woman in Botswana. They sit together in companionable silence as night falls over the country.