Sanger Rainsford is an experienced big game hunter from New York City who firmly believes the world is divided into two classes: the hunters and the hunted. He exhibits pride and self-importance, scoffing at the superstitions surrounding Ship-Trap Island and showing no sympathy for the prey he tracks. When forced into a survival situation, he relies on his quick wits and capacity for creative problem-solving under pressure.

General Zaroff is a wealthy, aristocratic former commander in the Cossack cavalry who lives on a remote jungle island. He projects the image of a highly educated gentleman, treating his guests to fine wine and tailored clothing. However, his cultivated manners mask a deep cruelty and an obsession with hunting that requires increasingly cunning prey.

Whitney is a sensitive and observant big game hunter traveling to the Amazon. Unlike his companion, he notices the jumpiness of the ship's crew and acknowledges that fear is a universal feeling shared by both humans and animals. He possesses a humbler worldview and is willing to consider the perspective of the creatures they pursue.

Ivan is a massive, heavily bearded man who serves as the muscle behind the operations on Ship-Trap Island. He is deaf and cannot speak, relying entirely on the orders of his employer to direct his actions. Having previously carried out whippings for the Great White Czar, he is accustomed to doing the bidding of powerful, ruthless men.