The Lady is the teenage daughter of a noble peer, traveling to her father's inauguration at Ludlow Castle. She acts as the embodiment of chastity, temperance, and Christian virtue. Grounded in her faith and rationality, she views the natural world through a lens of order and sobriety. Her deep moral convictions arm her against deceit, allowing her to see through false arguments that appeal strictly to physical appetites.

Comus is an evil sorcerer who haunts the wild wood with a coterie of monstrous followers. As the son of classical deities Bacchus and Circe, he represents chaos, debauchery, and the indulgence of base instincts. He wields a magical potion that overthrows human reason, reducing those who drink it to an animalistic state. Highly skilled at illusion, he relies on persuasive rhetoric and specious logic to disguise his sinful intentions as natural law.

The Attendant Spirit is a celestial being sent by Jove to watch over the noble children on their journey. To interact safely with mortals, he adopts the disguise of Thyrsis, the family's trusted shepherd. He acts as an agent of divine providence, providing the humans with heavenly knowledge and magical safeguards, such as the protective plant haemony, to combat the sorcery they encounter in the wilderness.

The older brother of the Lady is a highly rational and philosophically minded young noble. He maintains absolute confidence in his sister's safety, arguing that her inherent virtue and chastity function as an invincible spiritual armor. He interprets the world through classical and Christian doctrines, believing that a pure mind generates its own light to dispel external darkness.

The Lady's younger brother is practical and highly protective. Unlike his older sibling, he evaluates their situation through physical vulnerabilities rather than spiritual guarantees, worrying that his sister's status as an isolated maiden puts her at immediate risk from hostile elements. His perspective highlights unaided human reasoning in the face of uncertainty.

Sabrina is a water nymph and the presiding goddess of the river Severn. Originally a mortal who drowned, she evolved into a divine spirit associated with mercy, purification, and the protection of chaste virgins. She commands pure waters that possess the power to undo dark sorcery and liberate those trapped by physical enchantments.

The Earl is a noble peer assuming the role of Lord President of Wales. His castle at Ludlow Town represents civilization, order, and safety. His inauguration serves as the destination for his three children, driving the narrative action as they travel through the chaotic wilderness to reach his secure court.