Darwi Odrade is the Senior Security Mother for the Bene Gesserit and a descendant of the Atreides bloodline. Having been raised by foster parents whom she secretly still loves, she struggles to reconcile her innate capacity for affection with the Sisterhood's demands for strict emotional detachment. She possesses a keen prescient instinct and serves as a vital agent in analyzing the shifting political motives of rival factions.

Miles Teg is the 296-year-old former Supreme Bashar of the Bene Gesserit military forces. A Mentat and direct descendant of the Atreides line, he physically resembles his ancestor Duke Leto I and grounds his tactical brilliance in a firm sense of fairness and loyalty. He is called out of a peaceful farming retirement to oversee the defense and training of the Gammu Keep.

Alma Mavis Taraza serves as the Reverend Mother Superior, wielding ultimate authority over the Bene Gesserit Sisterhood. She maintains a highly disciplined, impenetrable exterior to mask the heavy emotional toll and loneliness of leadership. She orchestrates complex schemes, including the new ghola project, to secure the Sisterhood's survival against rising external threats from the Scattering.

The Ghola Duncan Idaho is a young artificially created replicant, grown by the Tleilaxu from the cells of the legendary Atreides swordmaster. Raised under strict Bene Gesserit supervision on Gammu, he represents the Sisterhood's twelfth attempt at an obscure, multi-generational design. He deeply resents his imprisonment and yearns to uncover his true identity by accessing his dormant original memories.

Sheeana Brugh is a fourteen-year-old native of the desert planet Rakis who miraculously survives a devastating sandworm attack that destroyed her entire village. She possesses an unprecedented ability to communicate with and command the giant worms without traditional tools. Her unique talents draw the intense scrutiny of the local priesthood and the Bene Gesserit, who view her as a crucial element in their long-term designs.

Tylwyth Waff acts as the Master of Masters, leading the secretive Bene Tleilax council. While he projects an outward appearance of subservience and inferiority, he carefully directs the production of gholas and shape-shifting Face Dancers to weaken his rivals. He secretly harbors profound Zensunni religious beliefs that guide his political maneuvers.

Lucilla is a young Bene Gesserit Reverend Mother assigned to the Gammu Keep. Bred specifically for her motherly disposition and genetic resemblance to Darwi Odrade, she is tasked with sexually imprinting the young ghola to secure his permanent loyalty to the Sisterhood. Over time, she experiences a growing internal conflict regarding the Sisterhood's emotional repression and her role as an expendable operative.

Patrin is a native of Gammu and serves as Miles Teg's fiercely devoted aide. Despite possessing the military skills for a much higher rank, he prefers to remain close to the common people and his trusted commander. He utilizes his intimate knowledge of Gammu's wilderness and history to assist in Teg's security operations.

Schwangyu is the cold and impersonal Bene Gesserit Reverend Mother commanding the Gammu Keep. She harbors deep suspicions about the ghola project, fearing that the Tleilaxu have sabotaged the boy or that the Sisterhood risks creating another uncontrollable tyrant. She treats her charges with harsh discipline.

Burzmali is the current Supreme Bashar of the Bene Gesserit military, having assumed the highest rank after Miles Teg's retirement. He is a highly capable tactician entrusted with executing Taraza's military strategies and locating missing personnel during periods of intense crisis.

Hedley Tuek serves as the High Priest of the Church of the Divided God on Rakis. He capitalizes on Sheeana's miraculous survival, framing her as a divine messenger to solidify his own political and religious authority over the local population and rival priests.

Stiros is an older, ambitious priest within the Rakian religious hierarchy. Skeptical of Sheeana's alleged divinity, he bitterly resents High Priest Tuek's authority and quietly plots to elevate his own family members to positions of supreme power.

Murbella is an Honored Matre, a member of the dangerous and vengeful female faction returning from the Scattering. She relies on immense physical prowess and specialized sexual techniques to subjugate men, viewing the inhabitants of the Old Imperium with predatory disdain.

Janet Roxbrough-Teg is a Bene Gesserit Reverend Mother descended from the Fish Speakers and the mother of Miles Teg. Though deceased, her unconventional, mildly heretical teachings continue to shape her son's profound Mentat abilities and moral compass.

Loschy Teg is a minor official in the CHOAM trade conglomerate and the father of Miles Teg. His union with Janet Roxbrough formed the foundation of one of the Bene Gesserit's greatest military minds.