Prof. Robert Langdon is an athletic, middle-aged art history and symbology professor at Harvard University. Possessing an eidetic memory and an extensive knowledge of history and the works of Dante Alighieri, he finds himself plunged into a deadly puzzle with no recollection of how he arrived in Europe. He relies on his academic expertise to decipher cryptic clues while evading armed pursuers.

Dr. Sienna Brooks is a young British doctor working in Florence who becomes swept up in Prof. Robert Langdon's escape. A former child prodigy with an extraordinarily high IQ, she possesses an advanced brain physiology that caused her to be ostracized in her youth. Resourceful and physically capable, she serves as Langdon's primary guide and protector as they cross the city.

Dr. Elizabeth Sinskey is the dedicated head of the World Health Organization. She views global health from a macroscopic perspective, prioritizing systemic issues like overpopulation and pandemic containment. She acts as a high-level authority figure attempting to manage a severe biological threat while utilizing a heavily armed tactical team to secure her objectives.

The Provost is the pragmatic, unnamed leader of The Consortium, an independent organization that provides covert operations and absolute secrecy to the highest bidder. Operating from his offshore command center, he adheres strictly to compartmentalized protocols and avoids passing moral judgment on his clients. He manages a vast network of agents and facilitators to execute a mysterious contract.

Dr. Bertrand Zobrist is an infamous biochemist and brilliant geneticist known for his radical views on the Population Apocalypse Equation. Believing that extreme measures are necessary to prevent a global environmental collapse, he operates as a driven and highly intelligent extremist. His legacy takes the form of cryptic, Dante-inspired puzzles left behind to protect a biohazard he calls "Inferno."

Christoph Brüder is the intense and highly capable leader of the Surveillance and Response Support team. Tasked with tracking down Prof. Robert Langdon, he utilizes advanced tracking equipment and coordinates a heavily armed tactical unit. He operates with military efficiency in a high-stakes manhunt across historical European cities.

Vayentha is a strong, spike-haired operative working as an assassin for The Consortium. Tasked with a critical mission involving Prof. Robert Langdon, she proves relentless and deadly. Her status within her organization relies entirely on her ability to successfully execute her assigned objectives without error.

Laurence Knowlton is a senior facilitator for The Consortium. Working from a cubicle on the mobile command center, he manages sensitive client data and automated uploads. He becomes increasingly uneasy as he reviews the disturbing contents of a client's scheduled video broadcast.

Ignazio Busoni is the esteemed curator of Il Duomo in Florence and an academic colleague of Prof. Robert Langdon. Nicknamed "il Duomino" due to his portly stature, he shares Langdon's deep appreciation for Dante Alighieri and assists him in investigating a historical artifact just before Langdon loses his memory.

Marta Alvarez is an employee at the Palazzo Vecchio who manages access to historical artifacts. Visibly pregnant and cheerful, she recognizes Prof. Robert Langdon from his visit the previous night, inadvertently helping him retrace his forgotten steps while remaining unaware of the danger surrounding him.

Dr. Ferris is a mysterious man wearing Plume Paris sunglasses who claims to be the individual who originally brought Prof. Robert Langdon to Florence. He suffers from a severe, spreading rash that he dismisses as an allergic reaction to local soaps, offering to help Langdon and Sienna travel to Venice to continue their search.

Dr. Marconi is an Italian-speaking doctor acting as Dr. Sienna Brooks's superior at the hospital. He is present when the assassin Vayentha breaches the facility and bravely attempts to block her entry.

Ettore Vio is the knowledgeable curator of St. Mark's Basilica. He is an old friend of Prof. Robert Langdon who generously assists him in deciphering historical clues related to the Fourth Crusade and the Venetian doge Enrico Dandolo.

Mirsat is an enthusiastic tour guide at the Hagia Sophia in Istanbul. He is highly respectful of Prof. Robert Langdon's academic reputation and eagerly provides the group with a private, after-hours tour of the ancient structure's historical tombs and architecture.