Sally is a ragdoll stitched together and stuffed with dead leaves who recently married Jack Skellington. She struggles with deep insecurity about her new role as the Pumpkin Queen of Halloween Town. Driven by a desire for independence and armed with a deep knowledge of potions, she accidentally uncovers a hidden door in the woods that unleashes a mysterious threat upon the holiday worlds.

Jack is the enthusiastically impulsive Pumpkin King of Halloween Town. He recently married Sally and cares deeply for her, though he remains somewhat oblivious to her internal struggles regarding her new royal status. He immerses himself in Halloween preparations until an unnatural slumber overtakes him and the rest of the town.

Doctor Finkelstein is a pale, large-headed scientist in Halloween Town who claims to have created Sally. He has long treated her as an object and servant, attempting to confine her to a subordinate role. His garden provides the medicinal herbs Sally relies upon for her potion-making.

The Sandman is a mysterious old man with a long beard and frizzy hair who floats just above the ground. Wrapped in a cloudy cloak, he spills sand from his pockets while crooning a lullaby. He prowls the silent streets, spreading a fine sand that places everyone he encounters into a deathlike, unbreakable sleep.

Albert is a ragdoll, just like Sally, who lives in the mysterious land of Dream Town. He serves as a governor of the pajama-clad community that harvests lavender and herds counting sheep. He takes an immediate, intense interest in Sally when she arrives seeking help.

Greta is a ragdoll and co-governor of Dream Town alongside Albert. She oversees a walled town designed to protect its dream sand factory from outside threats. Upon meeting Sally, she offers her shelter and displays an unexpected, fierce affection toward the visiting Pumpkin Queen.

Zero is a faithful ghost dog who resides in Halloween Town. He serves as a loyal companion to both Jack and Sally, sensing danger and providing comfort when others fail to understand Sally's anxieties. He accompanies Sally into the wilder parts of the woods, attempting to keep her safe.

Ruby Valentino is the tall, stately, and elegant queen of Valentine Town. Surrounded by heart candy and cherubs, her perfectly groomed appearance and polished confidence trigger deep feelings of inadequacy in Sally during a honeymoon visit.

The Vampire Prince is a prominent citizen of Halloween Town involved in holiday planning. He takes an aggressive interest in Sally's new royal status, dragging her into wardrobe fittings and insisting she adopt a traditional, imposing aesthetic.

Helgamine is a witch in Halloween Town who attempts to redesign Sally's life and appearance. She drapes the new queen in heavy black chiffon, entirely ignoring Sally's actual preferences and comfort.

Zeldaborn is a witch and resident of Halloween Town. She participates in the unwanted royal makeover for Sally before becoming one of the first visible victims of the mysterious sleeping sickness that strikes the community.

A quick and clever resident of St. Patrick's Town who manages to stay awake while his neighbors fall into a magical slumber. Preoccupied with finding a pot of gold at the end of a rainbow, he provides Sally with crucial information about the ancient doors and a lucky charm.

An elderly human queen whom Sally discovers sound asleep in the library at Buckingham Palace. Observing this ordinary woman in an extraordinary role helps the ragdoll reconcile her own identity with her position as a ruler.