Usagi is a cheerful, easily frightened 14-year-old girl living in Azabu-Juuban. She struggles academically and frequently bursts into tears when overwhelmed. After receiving a magical brooch, she gains the ability to transform into a warrior charged with protecting Earth and locating the legendary Silver Crystal.

Luna is a talking black cat bearing a crescent-moon mark on her forehead. She serves as a strategist for the newly awakened guardians and provides them with necessary items like transformation pens. She operates a hidden high-tech base beneath a local arcade.

Mamoru is a sarcastic prep school student who lost his memories in a childhood car accident. He operates under a masked persona to search for the Silver Crystal, hoping it will restore his past. His methods often put him at odds with the other magical warriors.

Ami is a quiet, highly intelligent student rumored to possess an IQ of 300. She attends an elite study-prep seminar and keeps to herself socially before meeting Usagi. She uses her analytical mind and water-based attacks to assist her team in battle.

Rei is a reserved shrine maiden with natural psychic abilities. Her traditional spiritual practices and visions isolate her from others, leading some locals to view her with suspicion. She utilizes fire-based powers to incinerate threats and protect her community.

Makoto is a tall transfer student with a reputation for getting into physical altercations. Despite her intimidating exterior and independence, she possesses a gentle nature and wears floral accessories. She commands lightning and nature-based attacks.

Queen Beryl is the ruthless leader of the Dark Kingdom who commands forces from a subterranean underground kingdom. She seeks the legendary Silver Crystal to gain absolute power and feed energy to a slumbering ancient force. She operates through her generals while prioritizing her own ambitions.

Jadeite is the Dark Kingdom's commander of the Far East. He employs modern technology, such as educational software and buses, to drain human energy for his ruler.

Nephrite is the Dark Kingdom's commander of North America. He utilizes cursed items like mannequins and bridal wear to trap victims and extract their energy.

Zoisite is a subordinate in the Dark Kingdom who organizes mass-media attacks. He uses cursed television broadcasts and video rentals to harvest energy from the population.

Kunzite is one of the four heavenly kings serving the Dark Kingdom. He provides strategic counsel to his ruler during their pursuit of the ultimate power source.

Queen Metalia is an ancient dark entity entombed centuries ago. She relies on harvested human energy to briefly awaken and demand the Silver Crystal.

Naru is a cheerful student whose family owns a high-end jewelry store. Her proximity to Usagi and her family's business make her an early target for energy-draining schemes.

Umino is a socially awkward student who often shares local rumors and urban legends. He possesses a wealth of information about pop culture and recent news events.

Motoki is a friendly young man who works at the arcade where the girls frequently gather. He is completely unaware of the secret headquarters located beneath his workplace.

Princess D is the heir to a foreign throne who visits Japan to display a mythical royal treasure. Her presence draws the attention of thieves and monsters attending a masquerade ball.

Professor Izono is an individual who broadcasts false information across the city. The television program serves as a mass brainwashing trap designed to harvest civilian energy.

Sailor V is a mysterious guardian of justice who frequently appears in the media and inspires a popular arcade game. She carries a crescent-moon mark on her forehead and fights crime from the shadows.

Mii is a young girl from the local neighborhood who boards the six o'clock bus. She becomes one of the hostages trapped in another dimension by the Dark Kingdom.